Amnis imagestream manual

Manual microscopy allows visual identification of nuclear translocation on a per-cell basis but an objective and statistically rigorous assessment is difficult to obtain. The Amnis® FlowSight® ImageStream This is a unique assay on Amnis® FlowSight® ImageStream.  · measurements. Manual microscopy allows visual identification of nuclear translocation on a per-cell basis, but an objective and statistically rigorous assessment is difficult to obtain. To overcome these problems, we used the Amnis ® ImageStream Imaging Flow .  · 1. Settings. All lasers of the ImageStream®X Mk II System are set to full power, including the scatter laser (Figure 1). Magnification is set to 60X, and the core size is reduced to 7 μm. Channels not used are deselected and ‘remove beads’ is unchecked. Samples are loaded and acquired for 2 minutes (or specific fixed time for all samples).

1. Detailed instructions on using the systems are availabl e in the INSPIRE™ User's Manual for the Amnis® br and ImageStream® or FlowSight® Instruments. 2. Use the following INSPIRE version: • ImageStreamX - or later • ImageStreamX Mark II - or later • FlowSight - or later 3. Set Illumination Settings. We show that the use of the Amnis AI software to score imagery acquired using the ImageStream ® compares well to manual microscopy and outperforms IDEAS ® feature-based analysis, facilitating. The Amnis ImageStream®X Mark II is an imaging flow cytometer equipped with 4 lasers. Imaging Flow Cytometry combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. The ImageStream® instrument produces multiple images of every cell directly in flow, including.

This manual provides instruction for using the Amnis IDEAS® application to analyze data files from the Amnis ImageStream cell analysis system. The manual is organized into the following sections: • Introducing the IDEAS Application—Describes the hardware and software requirements. INSPIRE® ImageStreamX®SystemSoftware User'sManual PartNumberRev:B www.doorway.ru Amnis History Timeline • Founded in Seattle in , by David Basiji and Bill Oryton • 1st generation ImageStream® launched in • 2nd generation ImageStream®X launched in • Lower cost FlowSight® launched in • Amnis acquired by Merck/Millipore late • 3rd generation ImageStream®X MkII launched in