Hplc operating manual

Acces PDF Knauer Hplc Operating Manual User’s Manual - Agilent Quick Setup Instructions for the Knauer HPLC-System 3 / 9 once flow is established, stop the pumping cycle. attach one end of the connecting capillary to the outlet of the 6-port valve and the other one to the separation column. hook outlet of separation column. adversely affect LC/MS results, enhancing or suppressing ionization. To minimize these matrix effects, Waters recommends you adopt the following measures: † Prior to the instrumental analysis, use appropriate sample pretreatment such as protein precipitation, liquid/liquid extraction. See the Advion user’s manual if you need to create a custom calibration for your analysis. 2. Solvent Phase Transitioning • It may be necessary to transition the HPLC system from one solvent system to another. Transitioning may be column specific so consult the column’s user manual before attempting any of the following www.doorway.ru Size: KB.

This Standard Operating Procedure provides guidance on the proper way to integrate chromatographic peaks. Integration of peak areas is commonly required to relate instrument response to component concentration in Gas and Liquid Chromatography. Chromatographic data systems can be set up to detect and measure peak areas automatically. This protocol addresses good operating procedures and the techniques necessary to validate system and method performance. Safety advisories Consult Appendix A for a comprehensive list of warning and caution advisories. Operating the ACQUITY UPLC system When operating the ACQUITY UPLC system, follow standard quality control. Agilent LC Rheodyne series Agilent Valves Stator Face Part No. Part No. Manual injector Manual injector Autosampler , Switching valve Agilent LC Rheodyne Series Agilent Valves Stator Face Part No. Part No.

LCAT The LCAT is a solvent delivery unit that possesses a high level of maintainability while delivering high performance. The ability to remove bubbles has been improved by modifying the pump-head structure and the flow line. Low-Pressure Gradient Unit The optional low-pressure gradient unit can be incorporated in the LC-/20AT. standard operating procedures. Waters contact information: Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide. Visit www.doorway.ru Telephone and fax From the USA or Canada, phone , or fax For other locations worldwide, phone and fax. the operating pressure? below the operating pressure. 6. Does solvent flow out of the purge valve Go to next question In-line filter blocked. Clean as per when opened? the pump manual instructions 7. Was the pump primed? Go to next question Prime as per the pump instruction manual 8. Is air visible in the solvent lines?.